Screening of stool samples by Dot-Blot method. (A) Linear map of EhRI episome (24.5 kb) showing the position of HMe probe (4.5 kb in size) common for both E. histolytica and E. dispar), E - EcoR1 site and H- Hind III site; rDNA I and rDNA II represent two inverted repeats of transcription units with various restriction sites and repeats (B) Representative figure of Dot-blot analysis of stool sample using HMe probe. Rows 1 to 6 (column A-D) represent spots of DNA from stool samples. About 20 ng of DNA was loaded on each spot in triplicate on nylon membrane. Row 7 was blank. Row 8 (column A) E. histolytica HM1: IMSS genomic DNA as positive control; (column B) E. dispar SAW760 genomic DNA as positive control; (column C) E.Coli DH5α as negative control; (column D) Plasmid with cloned HMe as positive control. All samples were loaded in triplicate. Experimental details are provided in material and methods.
Verma et al. BMC Microbiology 2012 12:183 doi:10.1186/1471-2180-12-183