Table 1

Strains and plasmids used in this study

Strain or plasmid

Relevant genotype; phenotype

Reference or source


S. aureus


Newman

Clinical isolate, ATCC 25904, natural saeS constitutive mutant

[21,22]


BS304

Newman ΔesxA, markerless deletion

This study


SM148

Newman Δ(yabJ-spoVG)::erm(B); Emr

[8]


IK184

Newman Δ(rsbUVW-sigB)::erm(B); Emr

[23]


MS64

Newman sigB1(Am); Tcr

[24]


SM99

Newman ΔarlR::cat; Cmr

[9]


BS310

Newman Δagr::ermB; Emr

This study


KS186

Newman Δagr::tet(M); Tcr

[25]


BS309

Newman ΔsarA::ermB; Emr

This study


LR15

Newman ΔsarA::tet(L); Tcr

L. Reutimann


BB1002

Newman mec, MRSA derivative, Mcr

[26]


BS307

BB1002 ΔesxA, markerless deletion

This study


NM143

Newman GISA derivative, in vitro selected mutant; Ter

[27]


BS308

NM143 ΔesxA, markerless deletion

This study


E. coli


DH5α

F-Φ80d/acZΔM15 recA1

Invitrogen


Plasmids


pKOR1

E. coli-S. aureus shuttle vector for markerless deletions using the counter selection system

[28]


pAC7

Expression plasmid containing the PBAD promoter and the araC gene; Cmr

[29]


pAC7-sigB

pAC7 with a 0.75 kb fragment containing the gene sigB from S. aureus Col; Cmr

[30]


pBus1

E. coli-S. aureus shuttle plasmid with multicloning site from pBluescript II SK (Stratagene) and the rrnT14 terminator sequence from pLL2443; Tcr

[31]


pyabJ

pBus1 containing a bacA promoter-yabJ ORF fusion construct; Tcr

[10]


pspoVG

pBus1 containing a bacA promoter-spoVG ORF fusion construct; Tcr

[10]


pyabJspoVG

pBus1 containing a bacA promoter-yabJ-spoVG operon fusion construct; Tcr

[10]


pSP-luc+

Firefly luciferase casette vector; Apr

Promega


pesxAp-luc+

pBus1 containing an esxAp-luc+ fusion fragment; Tcr

This study


pesxApΔσA-luc+

pesxAp-luc+ with deletion of the σA promoter

This study


pesxApΔσB-luc+

pesxAp-luc+ with deletion of the σB promoter

This study


pSB40N

Promoter probe plasmid; Apr

[29]


pasp23p

pSB40N with a 0.6 kb fragment covering the asp23 promoter region fused to the reporter gene lacZα; Apr

[30]


pesxAp

pSB40N with a 0.5 kb fragment covering the esxA promoter region fused to the reporter gene lacZα; Apr

This study


pyabJp

pSB40N with a 0.4 kb fragment covering the yabJ promoter region fused to the reporter gene lacZα; Apr

This study


pSTM07

pSB40N with a 0.37 kb fragment covering the capA promoter region fused to the reporter gene lacZα; Apr

[9]


a Abbreviations are as follows: Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Mcr, methicillin resistant; Tcr, tetracycline resistant; Ter, teicoplanin resistant.

Schulthess et al. BMC Microbiology 2012 12:17   doi:10.1186/1471-2180-12-17

Open Data