Open Access Highly Accessed Research article

Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain

Nathan J Hare1, Nestor Solis1, Christopher Harmer2, N Bishara Marzook1, Barbara Rose2, Colin Harbour2, Ben Crossett1, Jim Manos2 and Stuart J Cordwell134*

Author Affiliations

1 School of Molecular Bioscience, The University of Sydney, Sydney 2006, Australia

2 Discipline of Immunology and Infectious Diseases, School of Medical Sciences, The University of Sydney, Sydney 2006, Australia

3 Discipline of Pathology, School of Medical Sciences, The University of Sydney, Sydney 2006, Australia

4 School of Molecular Bioscience, Building GO8, Maze Crescent, The University of Sydney, Sydney 2006, Australia

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BMC Microbiology 2012, 12:16  doi:10.1186/1471-2180-12-16

Published: 22 January 2012

Additional files

Additional file 1:

Growth curves for P. aeruginosa AES-1R, PAO1 and PA14 grown to stationary phase in LB medium. Dotted line and *, harvest time for PAO1 and PA14; #, for AES-1R.

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Additional file 2:

Table containing identification of differentially abundant proteins in P. aeruginosa AES-1R compared to PAO1 and PA14 using 2-DE.

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Additional file 3:

Table containing identification of differentially abundant proteins in P. aeruginosa AES-1R compared to PAO1 and PA14 using iTRAQ.

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Additional file 4:

Figure showing overlap of identified and quantified proteins by 2-DE and 2-DLC/MS with iTRAQ. Table showing relative abundance changes for 22 proteins quantified by both 2-DE and iTRAQ.

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Additional file 5:

Protein sequence alignment of Flagellin (FliC/FlaA) of P. aeruginosa strains used in this study (AES_1954, PA1092, and PA14_50290) and including an additional sequence from strain PAK with a known type A flagellin. The flagellin sequence of strain AES-1R has higher sequence similarity with the shorter A type flagellin of strain PAK (95%), while the type B flagellins of strains PA14 and PAO1 are almost identical with only a single amino acid difference.

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