Open Access Highly Accessed Research article

Storage conditions of intestinal microbiota matter in metagenomic analysis

Silvia Cardona1, Anat Eck1, Montserrat Cassellas1, Milagros Gallart1, Carmen Alastrue1, Joel Dore2, Fernando Azpiroz1, Joaquim Roca3, Francisco Guarner1 and Chaysavanh Manichanh1*

Author Affiliations

1 Digestive System Research Unit, Vall d’Hebron Institut de Recerca, Ciberehd, 08035, Barcelona, Spain

2 Institut National de Recherche Agronomique, Micalis UMR1319, Domaine de Vilvert, 78352, Jouy-en-Josas, France

3 Molecular Biology Institute of Barcelona, IBMB-CSIC, 08028, Barcelona, Spain

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BMC Microbiology 2012, 12:158  doi:10.1186/1471-2180-12-158

Published: 30 July 2012

Additional files

Additional file 1:

Table S1. Detailed taxonomy assignment at the species level of the 24 samples. The taxonomy analysis is based on alignment performed using PyNast against Silva 108 release database and OTUs assignment using blast and the Silva 108 release taxa mapping file.

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Additional file 2:

Figure S1. Taxonomy analysis at the phylum level of the 24 samples based on alignment performed using PyNast against Silva 108 release database and OTUs assignment using blast and the Silva 108 release taxa mapping file.

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Additional file 3:

Supplementary Methods. Detailed description of extraction of total RNA from fecal samples.

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