Table 2

Immunoreactive proteins of A.fumigates identified by MALDI-TOF-MS

Spot no.

Accession No. (GenBank)

Organism

Protein name

Peptides matched

Sequence coverage(%)

Mascot score

BLAST score (E-value)

Theoretical pI/Mr(kDa)

Probable functions


1A-1H

GI:71001112

Aspergillus fumigatus Af293

secreted dipeptidyl peptidase DppV

26

33

135

1.60E-08

5.59/79.7

Metabolism of dipeptides

2A-2M

GI:70992029

Aspergillus fumigatus Af293

thioredoxin reductase GliT

20

54

149

6.30E-10

5.44/36.2

Provide self-protection to A. fumigatus

3

GI:159123228

Aspergillus fumigatus A1163

FAD dependent oxidoreductase, putative

25

44

173

2.50E-12

5.94/51.5

Oxidoreductase

4

GI:70989411

Aspergillus fumigatus Af293

fumarylacetoacetate hydrolase FahA

13

37

85

0.0015

5.95/46.9

Phenylalanine catabolism, Tyrosine catabolism

5

GI: 119492487

Neosartorya fischeri NRRL 181

aspartyl aminopeptidase

20

40

98

8.90E-05

6.03/53.9

proteolysis, tissue invasion

6A-6B

GI: 70992355

Aspergillus fumigatus Af293

aldehyde dehydrogenase AldA

25

54

171

4.00E-12

6.30/61.4

Alcohol metabolism

7

GI: 71002030

Aspergillus fumigatus Af293

aromatic aminotransferase Aro8

19

52

145

3.10E-08

5.96/58.3

Aromatic aminoacid family metabolic process

8A-8B

GI: 70999466

Aspergillus fumigatus Af293

fructose-bisphosphate aldolase, class II

19

62

137

9.90E-09

5.55/39.9

Glycolysis, Carbohydrate degradation

9

GI: 119499942

Neosartorya fischeri NRRL 181

G-protein comlpex beta subunit CpcB

18

59

130

5.00E-08

6.06/35.3

Receptor signaling, intracellular signal transduction pathways, and protein synthesis

10

GI: 71001310

Aspergillus fumigatus Af293

actin cytoskeleton protein (VIP1)

13

40

86

0.0013

5.93/28.3

Component of cytoskeleton

11

GI: 159129975

Aspergillus fumigatus A1163

phytanoyl-CoA dioxygenase family

15

64

109

6.30E-06

6.08/33.7

Oxidization

12

GI: 70988713

Aspergillus fumigatus Af293

pectate lyase A

13

44

96

0.00014

6.23/33.8

Carbohydrate metabolism, cell wall biogenesis/degradation

13

GI: 71001408

Aspergillus fumigatus Af293

urate oxydase UaZ

12

32

80

0.0052

7.24/34.1

Metabolism of urate

14

GI: 70986899

Aspergillus fumigatus Af293

malate dehydrogenase, NAD-dependent

23

70

258

7.90E-21

9.08/35.8

Cellular carbohydrate metabolic process

15

GI: 169764553

Aspergillus oryzae RIB40

hypothetical protein

13

41

92

2.90E-04

6.21/35.3

unknown

16A-16B

GI: 70982195

Aspergillus fumigatus Af293

3-hydroxybutyryl-CoA dehydrogenase

15

44

90

0.00052

6.33/36.0

Fatty acid metabolic process

17

GI: 121711615

Aspergillus clavatus NRRL 1

proteasome component Pre8, putative

12

44

80

0.0052

5.55/30.1

Proteolysis involved in cellular protein catabolic process


Shi et al. BMC Microbiology 2012 12:11   doi:10.1186/1471-2180-12-11

Open Data