Figure 3.

SDS-PAGE and Western blot analysis of the recombinant thioredoxin reductase GliT (TR) of A. fumigatus. (A): SDS-PAGE analysis of the recombinant TR expressed in Escherichia coli BL21. Lane M, molecular weight marker; lane 1, pET28a -TR in E. coli BL21, 1 mM isopropyl-β- D - thiogalactoside induced for 5 h; lane 2, pET28a-TR in E. coli BL21, not induced; lane 3, purified recombinant TR; (B): Western blot analysis of the purified recombinant TR with sera of 6 patients with proven IA, pooled control patients, and monoclonal mouse anti-His antibody. lanes 1-6, Western blot of purified recombinant TR with sera from 6 patients with proven IA; lane 7, Western blot of purified recombinant TR with sera of pooled control patients; lane 8, Western blot of purified recombinant TR with monoclonal mouse anti-His antibody.

Shi et al. BMC Microbiology 2012 12:11   doi:10.1186/1471-2180-12-11
Download authors' original image