Table 1

Bacterial strains and plasmids
Bacterial strain Properties or Genotype Reference or source
    E. coli
TG2 supE hsdΔ5 thiΔ (lac-proAB) F' Δ(srl-recA)306::Tn10(Tcr) lacZΔM15 [31]
BL21(DE3)pLysS F-ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (Cmr) Novagen
    C. metallidurans
CH34 Zn, Cd, Co, Pb, Cu, Hg, Ni and Cr resistance [6]
AE104 Plasmid-cured C. metallidurans strain- sensitive to toxic metals [6]
Plasmid Description Reference or source
pET32LIC Apr Overexpression plasmid for ligation-independent cloning Novagen
pET32LIC pbrR AprpbrR cloned into pET32LIC This study
pMa5/8 Apr Cms Mutagenesis vector [32]
pMc5/8 Aps Cmr Mutagenesis vector [32]
pMaPbrR/PpbrA Apr Cms Mutagenesis vector with pbrR/PpbrA cloned in to it This study
pMOL1139 Kmr, The pbr operon cloned into plasmid pRK415 B. Borremans
pMU2385 Tpr 13.3 kb low copy number lacZ reporter plasmid [33]
pMUPpbrA Tpr pMU2385 containing the PpbrA promoter directing lacZ transcription This study
pMUPpbrA-1 Tpr pMU2385 containing the PpbrA promoter with a 1 bp deletion This study
pMUPpbrAcon Tpr As pMUPpbrA, but −10 sequence changed to E. coli consensus This study
pMUPpbrAmer Tpr As pMUPpbrA, but −10 sequence changed to mer promoter This study
pMUPbrR/PpbrA Tpr, pMU2385 containing pbrR, PpbrA ΔpbrA directing lacZ transcription This study
pMUPbrRC14S/PpbrA As pMUPbrRPpbrA, but PbrR C14S This study
pMUPbrRC55S/PpbrA As pMUPbrRPpbrA, but PbrR C55S This study
pMUPbrRC79S/PpbrA As pMUPbrRPpbrA, but PbrR C79S This study
pMUPbrRC114S/PpbrA As pMUPbrRPpbrA, but PbrR C114S This study
pMUPbrRC132S/PpbrA As pMUPbrRPpbrA, but PbrR C132S This study
pMUPbrRC134S/PpbrA As pMUPbrRPpbrA, but PbrR C134S This study
pMUPbrRC132,134 S/PpbrA As pMUPbrRPpbrA, but PbrR C132S/C134S This study
pUC21 Apr, high copy number cloning vector; ColE1 replicon [34]
pUK21 Kmr, intermediate copy number cloning vector; p15A replicon [34]
pUK21pbr1 Kmr, HindIII/SalI pbrR/PpbrApbrA from pMOL1139 cloned into pUK21 This study

Hobman et al.

Hobman et al. BMC Microbiology 2012 12:109   doi:10.1186/1471-2180-12-109

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