Table 2

Specific inhibition using dilutions of cell-free culture filtrates from Pseudomonas syringae pv.syringae UMAF0158 and its derived miniTn5 and insertion mutants grown in liquid minimal medium (PMS).

Bacterial strains

Mangotoxin production

Dilutions of cultures filtratesa


1:1

1:2

1:4

1:8

+ ornithine


Wild type

UMAF0158

+

21.7 ± 0.4

18.2 ± 0.4

13.7 ± 0.4

9.5 ± 0.5

< 7

miniTn5 mutants

UMAF0158-3νH1

-

< 7

< 7

< 7

< 7

< 7

UMAF0158-6νF6

-

< 7

< 7

< 7

< 7

< 7

pCG2-6 complementation

UMAF2-6-3H1

+

19.0 ± 1.0

15.5 ± 0.5

13.5 ± 0.5

9.5 ± 0.5

< 7

UMAF2-6A

+

19.0 ± 0.7

16.2 ± 0.4

12.7 ± 1.3

10.5 ± 0.5

< 7

Insertion mutants

UMAF0158::ORF1

+

20.2 ± 1.3

17.0 ± 0.7

14.7 ± 0.8

11.0 ± 0.8

< 7

UMAF0158::ORF2

+

19.7 ± 1.5

16.2 ± 0.8

12.2 ± 1.1

< 7

< 7

UMAF0158::mgoB

+

17.7 ± 0.8

14.2 ± 0.8

12.0 ± 0.8

< 7

< 7

UMAF0158::mgoC

-

< 7

< 7

< 7

< 7

< 7

UMAF0158::mgoA

-

< 7

< 7

< 7

< 7

< 7

UMAF0158::mgoD

-

< 7

< 7

< 7

< 7

< 7

pLac complementation

UMAF0158-6νF6 containing pLac56

+

19.2 ± 0.4

15.7 ± 0.8

12.7 ± 1.2

< 7

< 7

UMAF0158-6νF6 containing pLac6

-

< 7

< 7

< 7

< 7

< 7


The inhibition analysis was performed by Escherichia coli growth inhibition test

a) Toxic activity is expressed as diameter of inhibition zone (in mm). Average and standard deviation values were obtained from three replicate of three independent experiments

Arrebola et al. BMC Microbiology 2012 12:10   doi:10.1186/1471-2180-12-10

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