Table 1

Characterization of disrupted genes surrounding the mgo operon in derivates miniTn5 and insertional mutants from the wild type Pseudomonas syringae pv.syringae UMAF0158 mangotoxin producer

Bacterial strains

ORF disrupted

Mangotoxin productiona

Putative homology of disrupted gene

Comparison ncl-nclb with Pss B728a


% of identity

gene name


miniTn5 mutantsc

UMAF0158-3νH1

mgoC

-

Conserved hypothetical protein

95

Psyr_5010

UMAF0158-6νF6

mgoA

-

Nonribosomal peptide synthetase

93

Psyr_5011

Insertional mutants

UMAF0158::ORF0

ORF0

+

HAD hydrolase

92

Psyr_5006

UMAF0158::ORF1

ORF1

+

Aldo-keto oxidoreductase

98

Psyr_5007

UMAF0158::ORF2

ORF2

+

Transcriptional regulator GntR family

97

Psyr_5008

UMAF0158::mgoB

mgoB

(+)

Haem-oxigenase-likee

96

Psyr_5009

UMAF0158::mgoC

mgoC

-

p-aminobenzoate N-oxygenase AurFe

95

Psyr_5010

UMAF0158::mgoA

mgoA

-

Nonribosomal peptide synthetase

93

Psyr_5011

UMAF0158::mgoD

mgoD

-

Poliketide_cyc2d

94

Psyr_5012


a) Presence of inhibition halo around the bacterial growth point in E. coli growth inhibition test. -: absence of inhibition halo, +: presence of inhibition halo, (+): slight mangotoxin production (smaller and opaque halo)

b) ncl-ncl is used as abbreviation of nucleotide-nucleotide

c) miniTn5 mutants defective in mangotoxin production obtained in a previous work [15]

d) Putative function by family domains searches

Arrebola et al. BMC Microbiology 2012 12:10   doi:10.1186/1471-2180-12-10

Open Data