Organisation of the DNA cloned into pCG2-6 and the locations of the insertional and miniTn5 mutants used in this study. pCG2-6 contains an 11,103-bp insert of chromosomal DNA derived from Pseudomonas syringae pv. syringae UMAF0158 (GenBank accession number DQ532441). The site of insertion or miniTn5 within the UMAF0158-3γH1 and UMAF0158-6γF6 mutants (▼)  as well as the design of the insertional mutants (↑) generated in the current study are indicated. The predicted sites of the putative promoters (►) and transcriptional terminators (○) are indicated. The putative mgo operon involved in mangotoxin production by UMAF0158 is illustrated by grey arrows. Each gene studied in this study was given a specific name. The ORFs upstream of the mgo operon are illustrated by white arrows, and the 5S and 23S ribosomal RNAs are indicated by black arrows.
Arrebola et al. BMC Microbiology 2012 12:10 doi:10.1186/1471-2180-12-10