Open Access Research article

Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

Eva Arrebola1*, Víctor J Carrión2, Francisco M Cazorla2, Alejandro Pérez-García2, Jesús Murillo3 and Antonio de Vicente2

Author Affiliations

1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Estación Experimental La Mayora, Algarrobo-Costa, 29750 Málaga, Spain

2 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC). Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Unidad Asociada al CSIC, Campus de Teatinos, 29071 Málaga, Spain

3 Laboratorio de Patología Vegetal, ETS de Ingenieros Agrónomos, Universidad Pública de Navarra, 31006 Pamplona, Spain

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BMC Microbiology 2012, 12:10  doi:10.1186/1471-2180-12-10

Published: 17 January 2012

Additional files

Additional file 1:

Figure S1. Analysis of the plasmid integration in UMAF0158::mgoB. The PCR was performed using the M13F primer located in the lacZ gene of the pCR2.1 cloning vector and the ORF4204R primer located in the 5'-end of mgoC. Lane L: HyperLadder I (Bioline), lane 2: UMAF0158::mgoB, lane 3: UMAF0158, lane 4: negative control of the PCR reaction.

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Additional file 2:

Table S1. The annealing position and the sequence of the utilized primers in RT-PCR experiments.

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