Table 1

Changes in the gene expression of C. neoformans H99 cells exposed to FLC

BROAD ID (CNAG_*****)

C. n. gene name

S. c. gene name

Description

Fold change


Ergosterol biosynthesis

04804

SRE1

Sterol regulatory element-binding protein 1

+ 4.04

01737

ERG25

C-4 methyl sterol oxidase

+ 3.95

00854

ERG2

C-8 sterol isomerase

+ 3.47

02896

ERG13

Hydroxymethylglutaryl-CoA synthase

+ 3.03

06644

ERG5

C-22 sterol desaturase

+ 2.50

00040

ERG11

ERG11

Lanosterol 14 alpha-demethylase

+ 2.47

06829

ERG1

Squalene monooxygenase

+ 2.37

00519

ERG3

C-5 sterol desaturase

+ 2.21

01129

ERG7

Lanosterol synthase

+ 2.09

Transport

04632

FUR4

Uracil permease

+ 5.87

07448

DUR3

Urea transporter

+ 4.78

04758

MEP2/AMP2

Ammonium transporter

+ 3.78

06652

DAL5

Allantoate permease

+ 2.83

01742

AQY1

Water channel

+ 2.73

07902

CAN1

Amino acid transporter

+ 2.52

01960

YMR279C

Efflux protein EncT

+ 2.47

06338

PDR15

ABC transporter PMR5

+ 2.37

04898

ATR1

MFS transporter

+ 2.37

00284

YOR378W

Efflux protein EncT

+ 2.36

00097

ITR1

ITR1

+ 2.26

00895

ZRT1

Low-affinity zinc ion transporter

+ 2.20

04210

MPH2

Sugar transporter

+ 2.15

04617

OPT2

Small oligopeptide transporter

+ 2.11

05592

PMR1

Calcium-transporting ATPase

+ 2.06

01059

YBR241C

Vacuolar membrane protein

+ 2.02

00904

AZR1

Aflatoxin efflux pump AFLT

- 2.10

01769

AGC1

Mitochondrial inner membrane protein

- 2.16

04142

FEN2

Tartrate transporter

- 2.17

04567

TPO2

Drug transporter

- 2.22

05387

HXT5

Galactose transporter

- 2.28

02355

YEA4

UDP-N-acetylglucosamine transporter

- 2.30

05994

FLR1

Multidrug transporter

- 2.35

02733

STL1

Hexose transport-related protein

- 2.46

03794

YBR287W

Endoplasmic reticulum protein

- 2.58

00815

SIT1

Siderochrome-iron (Ferrioxamine) uptake transporter

- 2.92

01354

TNA1

Transporter

- 3.39

02104

SFH5

SFH5

Phosphatidylinositol transfer protein SFH5

- 4.54

07695

UGA4

Gamma-aminobutyric acid transporter

- 5.16

00749

YIL166C

Transporter

- 5.65

02083

ARN2

Siderochrome-iron transporter

- 9.48

Cell wall maintenance

02217

CHS7

Chitin synthase 7

+ 3.62

06336

BGL2

Glucan 1,3 beta-glucosidase protein

+ 2.61

03326

CHS2

Chitin synthase 2, CHS2

+ 2.20

01239

CDA3

CDA2

Chitin deacetylase

- 4.35

Capsule biosynthesis

03644

CAS3

CAS3p

+ 12.16

01489

CAS9

YJL218W

Putative O-acetyl transferase

- 3.84

Lipid and fatty acid metabolism

06085

PLB1

PLB1

Phospholipase B

+ 2.18

06623

MIOX

Myo-inositol oxygenase

+ 2.12

03128

ECM38

Lincomycin-condensing protein lmbA

- 2.01

00424

PCT1

Choline-phosphate cytidylyltransferase

- 2.02

05042

CAT2

Carnitine acetyltransferase

- 2.10

02000

FOX2

Short-chain dehydrogenase

- 2.95

00834

PSD2

Phosphatidylserine decarboxylase

- 3.10

02968

PLC2

Phospholipase C-2

- 4.11

Cell stress

03400

GRE2

Oxidoreductase

+ 3.54

05256

CTA1

Catalase 2

+ 2.81

02440

HSC82

Cation-transporting ATPase

+ 2.54

01750

HSP70

SSA1

Heat shock protein 70

+ 2.48

06917

TSA3

PRX1

Thiol-specific antioxidant protein 3

+ 2.09

03185

LOT6

Low temperature-responsive protein

+ 2.05

04622

SNG1

Response to drug-related protein

- 2.17

00575

CTT1

Catalase

- 2.21

01464

FHB1

YHB1

Flavo-haemoglobin

- 2.32

Amino acid metabolism

02284

PDA1

Branched-chain alpha-keto acid dehydrogenase E1-alpha subunit

+ 2.42

04862

GLT1

Glutamate synthase (NADH)

+ 2.39

04017

MXR2

Protein-methionine-R-oxide reductase

+ 2.32

01231

CAR1

Arginase

+ 2.27

03828

ARO8

Aromatic amino acid aminotransferase I

+ 2.26

06540

ILV3

Dihydroxy-acid dehydratase

+ 2.18

00247

LYS9

Saccharopine dehydrogenase (NADP+, L-glutamate-forming)

+ 2.02

02270

MET2

Homoserine O-acetyltransferase

- 2.11

01076

UGA1

4-aminobutyrate transaminase

- 2.18

00237

LEU1

3-isopropylmalate dehydratase

- 2.27

01264

LYS12

Isocitrate dehydrogenase

- 2.31

00879

GDH2

Glutamate dehydrogenase

- 2.33

04467

UGA2

Succinate-semialdehyde dehydrogenase (NAD(P)+)

- 2.83

02851

GLY1

Threonine aldolase

- 3.04

02049

PUT1

Proline dehydrogenase

- 5.74

05602

PUT2

1-pyrroline-5-carboxylate dehydrogenase

- 6.65

Carbohydrate metabolism

06374

MAE1

Malic enzyme

+ 6.04

02225

CELC

EXG1

Cellulase

+ 3.99

02552

TKL1

Transketolase

+ 3.28

04025

TAL1

Transaldolase

+ 3.00

00696

AMS1

Alpha-mannosidase

+ 2.52

05913

MAL12

Alpha-glucosidase

+ 2.34

05113

ALD4

Aldehyde dehydrogenase (ALDDH)

+ 2.11

05264

YJL216C

Alpha-amylase AmyA

+ 2.08

03946

GAL1

Galactokinase

- 2.16

07752

GLF

UDP-galactopyranose mutase

- 2.23

04659

PDC1

Pyruvate decarboxylase

- 2.33

06924

SUC2

Beta-fructofuranosidase

- 2.57

00269

SOR1

Sorbitol dehydrogenase

- 2.62

00393

GLC3

GLC3

1,4-alpha-glucan-branching enzyme

- 2.93

07745

MPD1

ADH3

Mannitol-1-phosphate dehydrogenase

- 3.54

04217

PCK1

Phosphoenolpyruvate carboxykinase

- 8.67

04621

GSY1

Glycogen (Starch) synthase

- 11.00

04523

TDH3

Glyceraldehyde-3-phosphate dehydrogenase

- 11.45

Protein biosynthesis, modification, transport, and degradation

02389

YPK1

AGC-group protein kinase

+ 3.04

02531

FUS3

Mitogen-activated protein kinase CPK1

+ 2.91

03176

ERO1

Endoplasmic oxidoreductin 1

+ 2.36

05932

CPR6

CPR6

Peptidyl-prolyl cis-trans isomerase D

+ 2.35

01861

NAS6

Proteolysis and peptidolysis-related protein

+ 2.35

04635

PEP4

Endopeptidase

+ 2.31

06872

YKL215C

5-oxoprolinase

+ 2.27

05005

ATG1

ATG1

Serine/threonine-protein kinase ATG1

+ 2.20

00919

KEX1

Carboxypeptidase D

+ 2.13

04625

PRB1

Serine-type endopeptidase

- 2.01

00130

RCK2

Serine/threonine-protein kinase

- 2.12

04108

PKP1

Kinase

- 2.17

02327

YFR006W

Prolidase

- 2.28

02418

DED81

Asparagine-tRNA ligase

- 2.40

03563

DPS1

Aspartate-tRNA ligase

- 2.50

04275

OMA1

Metalloendopeptidase

- 2.50

02006

NTA1

Protein N-terminal asparagine amidohydrolase

- 2.75

03949

PHO13

4-nitrophenylphosphatase

- 3.32

TCA cycle

03596

KGD2

2-oxoglutarate metabolism-related protein

- 2.02

03920

IDP1

Isocitrate dehydrogenase (NADP+)

- 2.06

03674

KGD1

Oxoglutarate dehydrogenase (Succinyl-transferring)

- 2.52

00747

LSC2

Succinate-CoA ligase (ADP-forming)

- 2.70

07363

IDH2

Isocitrate dehydrogenase

- 2.80

01137

ACO1

Aconitase

- 2.99

07851

IDH1

Isocitrate dehydrogenase (NAD+), putative

- 3.80

Glycerol metabolism

06132

RHR2

Glycerol-1-phosphatase

+ 2.31

02815

GUT2

Glycerol-3-phosphate dehydrogenase

- 2.00

Nucleotide metabolism

05545

HNT2

Nucleoside-triphosphatase

+ 2.25

03078

NPP1

Type I phosphodiesterase/nucleotide pyrophosphatase family protein

+ 2.08

06489

ADO1

Adenosine kinase

- 2.08

00613

FCY1

Cytosine deaminase

- 2.69

Thiamin metabolism

03592

THI20

Phosphomethylpyrimidine kinase

- 2.51

Alcohol metabolism

05258

SMG1

Glucose-methanol-choline (GMC) oxidoreductase

+ 6.67

05024

SPS19

L-xylulose reductase

+ 2.53

06168

GNO1

SFA1

GSNO reductase

- 2.02

Carbon utilization

05144

CAN2

NCE103

Carbonic anhydrase 2

- 3.18

Cell cycle control

03385

PCL1

G1/s-specific cyclin pcl1 (Cyclin hcs26)

+ 2.37

02604

HOP1

Putative uncharacterized protein

+ 2.19

00995

MSC1

Meiotic recombination-related protein

- 3.63

Chromatin and chromosome structures

02115

NHP6B

Nonhistone protein 6

- 2.47

Transcription

01841

GLN3

Predicted protein

+ 5.72

02990

YOR052C

Nucleus protein

+ 2.16

04594

UGA3

PRO1 protein

- 2.01

05290

SPT3

Transcription cofactor

- 2.01

06495

RNH70

Ribonuclease H

- 2.06

05333

PUT3

Putative uncharacterized protein

- 2.14

02338

GIS2

DNA-binding protein hexbp

- 2.47

05479

ASG1

Putative uncharacterized protein

- 3.57

Signal transduction

03316

RDI1

Rho GDP-dissociation inhibitor 1

+ 2.07

00363

HHK5

SLN1

CnHHK5 protein

- 2.44

01262

GPB1

STE4

G-protein beta subunit GPB1

- 2.55

Oxidoreduction

04652

YLR460C

Enoyl reductase

+ 2.63

06035

ADH1

Alcohol dehydrogenase

+ 2.41

00605

ZTA1

Cytoplasm protein

+ 2.20

00038

SOR2

Alcohol dehydrogenase

+ 2.13

01954

YPR127W

Aldo/keto reductase

+ 2.09

02958

FET5

Ferroxidase

+ 2.06

02935

YMR226C

Oxidoreductase

- 2.01

01558

XYL2

Zinc-binding dehydrogenase

- 2.28

00876

FRE7

Ferric-chelate reductase

- 2.49

03168

MET10

Sulfite reductase (NADPH)

- 2.55

07862

YEL047C

Fumarate reductase (NADH)

- 2.58

03498

FRE2

Metalloreductase

- 2.85

03874

AIF1

Oxidoreductase

- 2.89

Other

00331

YMR210W

Anon-23da protein

+ 3.43

04934

TAR1

Temperature associated repressor

+ 2.37

05678

ADY2

Membrane protein

+ 2.28

00818

AGE2

AGD15

+ 2.23

04867

YJR054W

Vacuole protein

+ 2.22

06574

APP1

Antiphagocytic protein 1

+ 2.21

06482

AMD2

Amidase

+ 2.20

01252

TUM1

Thiosulfate sulfurtransferase

- 2.05

03452

AFG1

AFG1 family mitochondrial ATPase

- 2.16

05831

MMF1

Brt1

- 2.19

03991

YGR149W

Integral to membrane protein

- 2.39

02039

YPL264C

Integral membrane protein

- 2.46

02943

SLM1

Cytoplasm protein

- 2.49

06668

AIM38

Mitochondrion protein

- 2.61

00638

LSG1

GTPase

- 2.89

01653

CIG

Cytokine inducing-glycoprotein

- 3.26

04314

YEF1

NAD+ kinase

- 3.74

04690

FMP41

Mitochondrion protein

- 5.52


Genes that were found to be differentially expressed were ordered by expression level and categorized, if available, into functional groups as described in Materials and Methods. Results are presented as the mean fold-increase (symbol +) or -decrease (symbol -) of biological triplicates. Abbreviations: C. n., C. neoformans; S. c., S. cerevisiae.

Florio et al. BMC Microbiology 2011 11:97   doi:10.1186/1471-2180-11-97

Open Data