Table 1

Bacterial strains and plasmids used in this study

Strains or plasmids

Genotypes or descriptions

Sources


C. crescentus

LS107

syn-1000, bla-6, amps derivative of NA1000

Stephens et al. [45]

JG1172

syn-1000 bla-6 ΔfliX

Muir et al. [38]

SC1032

flbD198::Tn5

Ohta et al. [41]

E. coli

S17-1

Rp4-2, Tc::Mu, Km::Tn7

Simon et al. [46]

BL21(DE3)

F-ompT gal [dcm] [lon] hsdSB (rB- mB-; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene

Novagen

Plasmids

pX21b

derivative of pET-21b carrying histidine-tagged FliX under the control of T7 promoter, Apr

Muir & Gober [36]

pBBR1MCS

broad host range cloning vector, multicopy, Cmr

Kovach et al. [47]

pZXfliX

derivative of pBBR1MCS carrying PCR generated 894 bp BamHI-HindIII fragment containing fliX gene and its promoter

This study

pZX71A

derivative of pZXfliX, codon 71 cgcc→gcc

This study

pZX85K

derivative of pZXfliX, codon 85 ctg→aag

This study

pZXΔ117-8

derivative of pZXfliX, deletion of codon 117 and 118

This study

pZX130L

derivative of pZXfliX, codon 130 acc→ctg

This study

pZX136K

derivative of pZXfliX, codon 136 ctg→aag

This study

pfliX1

derivative of pBBR1-MCS expressing fliX 1, an allele of fliX carrying a point mutation at the sixteenth codon (R16G), a frame shift at codon 141, and an extended carboxyl terminus of 67 amino acids

Muir et al. [38]

pfliF/lacZ/290

fliF-lacZ transcriptional reporter vector, Tcr

Wingrove & Gober [48]

pfliK/lacZ/290

fliK-lacZ transcriptional reporter vector, Tcr

Gober & Shapiro [25]


Xu et al. BMC Microbiology 2011 11:89   doi:10.1186/1471-2180-11-89

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