Proteins bound to the sepharose beads coated with histidine-tagged FliX. Purified FliX-His was conjugated to sepharose beads prior to incubation with cell lysis of LS107. The bead complexes were boiled with the sample buffer and were subject to SDS-PAGE analysis. The identities of the five major bands were determined by mass spectrometry.
Xu et al. BMC Microbiology 2011 11:89 doi:10.1186/1471-2180-11-89