Identification and characterisation of a novel adhesin Ifp in Yersinia pseudotuberculosis
1 Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
2 School of Molecular and Medical Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK
3 School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK
BMC Microbiology 2011, 11:85 doi:10.1186/1471-2180-11-85Published: 28 April 2011
Additional file 1:
Amino acid alignment of Ifp from the four currently sequenced genomes of Y. pseudotuberculosis. Utilising the ClustalW program, the amino acid sequences of Y. pseudotuberculosis strains IP32953, IP31758, PB1 and YPIII were aligned.
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Additional file 2:
Growth curves from the temporal expression of Ifp and invasin assay. Within the Anthos Lucy1 combined photometer and luminometer, OD readings at 600 nm were taken at 30 minute intervals and used to construct these growth curves. Cultures were incubated at (A) 24°C (B) 28°C and (C) 37°C.
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