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Resolution: standard / high Figure 1.
E. chaffeensis RNA polymerase purification by employing heparin agarose column purification method. A) Silver-stained SDS-PAGE gel profile of heparin agarose purified fractions of
E. chaffeensis RNA polymerase. M, protein standards (kDa); C, E. chaffeensis crude lysate; W1, first wash fraction from the column; W2, second column wash; E1,
first elution fraction; E2, second elution fraction; P, pooled dialyzed fractions
of eluted fractions 3 to 6; Ec, E. coli holoenzyme from Epicenter® B) Western blot analysis of the proteins resolved in panel A with E. coli anti-sigma70 monoclonal antibody, 2G10.
Faburay et al. BMC Microbiology 2011 11:83 doi:10.1186/1471-2180-11-83 |
