E. chaffeensis RNA polymerase purification by employing heparin agarose column purification method. A) Silver-stained SDS-PAGE gel profile of heparin agarose purified fractions of E. chaffeensis RNA polymerase. M, protein standards (kDa); C, E. chaffeensis crude lysate; W1, first wash fraction from the column; W2, second column wash; E1, first elution fraction; E2, second elution fraction; P, pooled dialyzed fractions of eluted fractions 3 to 6; Ec, E. coli holoenzyme from Epicenter® B) Western blot analysis of the proteins resolved in panel A with E. coli anti-sigma70 monoclonal antibody, 2G10.
Faburay et al. BMC Microbiology 2011 11:83 doi:10.1186/1471-2180-11-83