Epifluorescence micrographs of fluorescent in situ hybridized tissue samples taken from neonates diagnosed with necrotizing enterocolitis. All the specimens were hybridized with a general bacterial probe (EUB338) tagged with fluorescein (green colour) and group specific probes tagged with Cy3 (red colour). A) Visualization of Enterobacteria (GAM42a). B) Visualization of Actinobacteria ( pB00182). C) Visualization of Clostridium butyricum ( S-S-C. butyricum-663) in the two neonates where pneumatosis intestinalis was verified by histopathology. D) Visualization of Clostridium perfringens (S-S-C.perfring-185-a-A-18) in neonate number 3 with pneumatosis intestinalis.The scale bar is 20 μm in all the micrographs.
Smith et al. BMC Microbiology 2011 11:73 doi:10.1186/1471-2180-11-73