Open Access Highly Accessed Research article

Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors

Chantal Quiblier1, Annelies S Zinkernagel2, Reto A Schuepbach3, Brigitte Berger-Bächi1 and Maria M Senn1*

Author Affiliations

1 Institute of Medical Microbiology, University of Zurich, Gloriastr. 32, 8006 Zurich, Switzerland

2 Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Raemistr. 100, 8091 Zurich, Switzerland

3 Surgical Intensive Care, University Hospital Zurich, University of Zurich, Raemistr. 100, 8091 Zurich, Switzerland

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BMC Microbiology 2011, 11:72  doi:10.1186/1471-2180-11-72

Published: 12 April 2011

Additional files

Additional file 1:

Figure S1 - SpA processing in strain Newman. Western blot analyses of (A) subcellular fractions of wild type grown to an OD600 of 3 and (B) of total extract from overnight cultures of wild type and spa mutant using goat anti-human IgA antibodies. Coomassie stained total protein is shown on the right as an indication of loading. SN, supernatant; CW, cell wall; CM, cell membrane; CP, cytoplasm.

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Additional file 2:

Table S1 - Primers used in this study.

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