Open Access Research article

Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coli BL21 (DE3)

Hendrik Waegeman1*, Joeri Beauprez1, Helena Moens1, Jo Maertens1, Marjan De Mey1*, Maria R Foulquié-Moreno2, Joseph J Heijnen3, Daniel Charlier2 and Wim Soetaert1

Author Affiliations

1 Centre of Expertise-Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium

2 Laboratory for Genetics and Microbiology, Department of Applied Biological Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium

3 Kluyver Laboratory for Biotechnology, Department of Biochemical Engineering, Delft University of Technology Julianalaan 67, 2628 BC Delft

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BMC Microbiology 2011, 11:70  doi:10.1186/1471-2180-11-70

Published: 11 April 2011

Additional files

Additional file 1:

Average carbon and redox balances for batch and chemostat cultures. This file may be accessed using Microsof Excel or OpenOffice Spreadsheet.

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Additional file 2:

Corresponding gene products of genes used in Figure 2. This file may be accessed using Microsof Word or OpenOffice Word Processor.

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Additional file 3:

BLAST analysis of the arcA gene. This file may be accessed using Microsof Word or OpenOffice Word Processor.

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