Table 1

E. faecalis strains and plasmids used in this study.

strain or plasmid

characterization

reference


strains


E. faecalis 12030

Clinical isolate, strong biofilm producer

[33]


E. faecalis ATCC 29212

Reference strain


E. faecalis 12030ΔbgsA

(EF2891) bsgA mutant

[5]


E. faecalis 12030ΔbgsB

bgsB deletion mutant

This study


E. faecalis 12030ΔbgsB_rec.

Reconstituted mutant

This study


Escherichia coli DH5α

Gram-negative cloning host


Escherichia coli TOP10

Gram-negative cloning host

Invitrogen



plasmids


pCASPER

Gram-positive, temperature-sensitive mutagenesis vector

[34]


pCRII-TOPO

Gram-negative cloning vector

Invitrogen


pCASPER/ΔbgsB

This study


pMAD/bsgB

This study


pMAD

oripE194ts, EmR, AmpR, bgaB

[35]


Theilacker et al. BMC Microbiology 2011 11:67   doi:10.1186/1471-2180-11-67

Open Data