Comparison of 1H-NMR spectra of LTA isolated from E. faecalis 12030 wt, 12030ΔbgsB, and 12030ΔbgsA. Comparison of integration values of fatty acid (FA) signals (-CH2- and -CH3) as an internal reference and anomeric proton signal of glucose (H1 Glc A and H1 Glc B) indicated that the glycerolphosphate polymer of LTA from 12030ΔbgsB and 12030ΔbgsA contains approximately four times more kojibiose. Comparison of the resonance signal of total alanine (-CH3 Ala) and fatty acid signals (-CH2- (FA) and -CH3 (FA)) revealed that LTA extracted from either mutant also contains more alanine residues. Gro - glycerol.
Theilacker et al. BMC Microbiology 2011 11:67 doi:10.1186/1471-2180-11-67