Table 2

Detection of RD2 element genes in Lancefield group C and G streptococci by PCR.

A. Detection of genes encoding putative extracellular proteins


Strain

M28_ Spy1306

M28_ Spy1307

M28_ Spy1308

M28_ Spy1325

M28_ Spy1326

M28_ Spy1332

M28_ Spy1336


GCS


15169

+

+

-

+

+

-

+


15170

+

+

-

-

+

-

-


15172

+

+

+

+

+

-

+


15173

+

+

-

+

+

-

+


15178

+

+

+

+

+

+

+


15181

+

+

-

+

+

-

+


GGS


15163

+

+

-

+

+

-

+


15164

+

+

-

+

+

-

+


15165

+

+

-

+

+

-

+


15166

+

+

-

+

+

-

+


15167

+

+

-

+

+

-

+


15168

+

+

-

+

+

-

+


15171

+

+

+

+

+

-

+


15174

+

+

+

+

+

+

+


15175

+

+

+

-

-

-

-


15176

+

+

-

+

+

-

+


15177

+

+

-

+

+

-

+


15179

+

+

-

+

+

-

+


15180

+

+

+

+

+

-

+


15182

+

+

+

+

+

+

+


B. PCR-tiling across the entire RD2 element. Example of the tiling across RD2 is presented in Figure 3. (+) PCR product present, (-) no product, * amplified fragment of different size than for strain MGAS6180


PCR-tiling fragment no.


Strain

group

1

2

3

4

5

6

7

8

9

10

11

12


6180

A

+

+

+

+

+

+

+

+

+

+

+

+


15178

C

-

+

+

+

+

-

-

+

-

-

+

-


15174

G

+(*)

+

+

+

+

+

-

+

+

+

+

-


15182

G

-

+

+

+

+

+

+

+

+

+

+

-


Sitkiewicz et al. BMC Microbiology 2011 11:65   doi:10.1186/1471-2180-11-65

Open Data