Figure 3.

Detection of RD2 transfer from donor strain MGAS6180 (emm28) to recipient strain MGAS10750 (emm4). Amplicons 1-12 generated by PCR tiling across the RD2 element. A. transconjugant; * denote amplicons encompassing deleted M28_Spy1325-1326 region that is replaced by spectinomycin resistance cassette; B. control with chromosomal DNA isolated from strain MGAS6180. M - 1 kb ladder (Invitrogen)

Sitkiewicz et al. BMC Microbiology 2011 11:65   doi:10.1186/1471-2180-11-65
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