Correlation between the microarray and the qRT-PCR data of 17 randomly selected genes. The ratios of changes in gene expression, from the microarray (each S. Typhimurium ORF was spotted in triplicate on the slide) and qRT-PCR experiments, for the arcA mutant relative to the WT were log2 transformed and linearly correlated. The genes selected and the primers used in qRT-PCR are listed in Table 2. Three amplifications of each of the 17 genes were made using 1:5:25 dilutions of the total RNA.
Evans et al. BMC Microbiology 2011 11:58 doi:10.1186/1471-2180-11-58