Table 1

Bacterial strains and plasmids used in this study

Strain or plasmid

Description

Source or reference


Strains

Pasteurella pneumotropica

ATCC 35149

Type strain, biotype Jawetz, isolated from mouse lung

ATCCa [50]

ATCC 12555

Biotype Heyl, isolated from mouse

ATCC [51]

CCUG 26450

Biotype Jawetz, isolated from gerbil

CCUGb

CCUG 26451

Biotype Jawetz, isolated from hamster

CCUG

CCUG 26453

Biotype Heyl, isolated from bird

CCUG

CCUG 36632

Biotype unknown, isolated from murine nose

CCUG

Escherichia coli

DH5α

Cloning strain

Stratagene

TOP10

Cloning strain

Invitrogen

BL21-AI

Protein expression strain

Invitrogen

TMU0812

BL21-AI ΔhlyE::Kmr

[13]

Plasmids

pTAC-1

Cloning vector, Apr

Biodynamics Laboratory

pENTR/SD/D-TOPO

Entry vector, Kmr

Invitrogen

pBAD-DEST49

Protein expression vector, N-terminal fusions to thioredoxin tag and C-terminal fusions to six-Histidine tag, Apr

Invitrogen

pET300/NT-DEST

Protein expression vector, N-terminal fusions to six-Histidine tag, Apr

Invitrogen

pTAC-PX3

0.5-kb pnxIIIA PCR fragment

This study

pBAD-Pnx3A

Entire pnxIIIA gene cloned into pBAD-DEST49

This study

pBAD-Pnx3A209

1.3-kb sequence of repeat 1 deleted from pBAD-Pnx3A

This study

pBAD-Pnx3A197

1.7-kb sequence of repeats 2 and 3 deleted from pBAD-Pnx3A

This study

pBAD-Pnx3A151

3.0-kb sequence of repeats 1, 2, and 3 deleted from pBAD-Pnx3A

This study

pET-Pnx3E

Entire pnxIIIE gene cloned into pET300/NT-DEST

This study


aAmerican Type Culture Collection

bCulture Collection of the University of Göteborg

Sasaki et al. BMC Microbiology 2011 11:55   doi:10.1186/1471-2180-11-55

Open Data