3-oxo-C6-HSL degradation by Acinetobacter GG2, Burkholderia GG4 and Klebsiella Se14 quorum quenching bacteria isolated from the ginger rhizosphere. Each rhizosphere bacterium or E. coli DH5α was incubated with 3-oxo-C6-HSL for 0, 24 h after which the cell culture supernatants were either spotted directly onto paper disks or acidified to pH 2 for 24 h to recyclize any ring opened 3-oxo-C6-HSL before spotting onto paper disks. These were placed on an agar plate overlaid with C. violaceum CV026 and incubated. A purple halo indicates the presence of 3-oxo-C6-HSL.
Chan et al. BMC Microbiology 2011 11:51 doi:10.1186/1471-2180-11-51