Open Access Highly Accessed Methodology article

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes

Esteban Martínez-García1, Belén Calles1, Miguel Arévalo-Rodríguez1 and Víctor de Lorenzo2*

Author Affiliations

1 Systems Biology Program. Centro Nacional de Biotecnología-CSIC (Calle Darwin 3, Campus de Cantoblanco), Madrid (E-28049), Spain

2 Biomedal SL (Av. Américo Vespucio, 5), Sevilla (E-41092), Spain

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BMC Microbiology 2011, 11:38  doi:10.1186/1471-2180-11-38

Published: 22 February 2011

Additional files

Additional File 1:

Supplementary Figures and Tables. Figure S1: Transposition time course during conjugative delivery of mini-Tn5 Km from pBAM1. Figure S2: Mini-Tn5 Km insertion mapping example. Figure S3: Consensus insertion site of the mini-Tn5 Km of pBAM1 in the genome of P. putida. Figure S4: Growth of P. putida wild type and an rpoN mutant strain in minimal medium. Table S1: Localization of mini-Tn5 Km transposon insertions within the P. putida KT2440 genome. Table S2: Details of the sites of insertion of mini-Tn5 Km in P. putida MAD1 white mutants. Table S3: Details of the sites of insertion of mini-Tn5 Km in P. putida MAD1 producing unusual white/blue patterns in X-gal plates. Table S4: Location of GFP-fusions generated with pBAM1-GFP within the P. putida KT2440 genome.

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