Table 3

Plasmid recombination frequency (Mean ± STD, × 10-3)

Strain

rec deletion

pYA4463a

pYA4590b

pYA4464+pYA4465c


S. Typhimurium

χ3761

None

1.55 ± 0.31

2.40 ± 0.54

2.88 ± 0.85

χ9833

ΔrecA62

1.07 ± 0.24

0.22 ± 0.07**

0.27 ± 0.07**

χ9070

ΔrecF126

1.14 ± 0.15

0.52 ± 0.07**

0.33 ± 0.09**

χ9072

ΔrecJ1315

1.87 ± 0.44

2.37 ± 0.21

1.10 ± 0.20**

χ9081

ΔrecJ1315 ΔrecF126

NAd

NA

0.35 ± 0.08**

χ9939

ΔrecF126 ΔrecA62

NA

0.41 ± 0.09**

0.35 ± 0.08**

χ9833(pYA5002)

ΔrecA62 (RecA+)

NA

2.50 ± 0.42

NA

χ9070(pYA5005)

ΔrecF126 (RecF+)

NA

2.00 ± 0.24

NA

S. Typhi Ty2

χ3769

None

4.69 ± 0.26

11.59 ± 2.61

4.20 ± 1.44

χ11159

ΔrecA62

1.32 ± 0.27**

0.60 ± 0.19**

3.37 ± 0.96

χ11053

ΔrecF126

0.51 ± 0.06**

0.57 ± 0.09**

6.19 ± 2.71

χ11134

ΔrecF1074

0.45 ± 0.05**

0.52 ± 0.17**

16.28 ± 2.64**

χ11194

ΔrecJ1315

1.69 ± 0.26**

4.88 ± 1.56**

2.31 ± 0.90

χ11053(pYA5005)

ΔrecF126 (RecF+)

2.52 ± 0.18

NA

NA

χ11053(pYA5006)

ΔrecF126 (RecF+)

1.71 ± 0.68

NA

NA

χ11159(pYA5002)

ΔrecA62 (RecA+)

NA

14.35 ± 2.44

NA

χ11053(pYA5006)

ΔrecF126 (RecF+)

NA

2.86 ± 0.59

NA

S. Typhi ISP1820

χ3744

None

4.93 ± 0.67

13.10 ± 1.23

4.22 ± 0.25

χ11133

ΔrecF1074

0.65 ± 0.26**

0.71 ± 0.06**

5.38 ± 0.58

S. Paratyphi A

χ8387

None

2.70 ± 0.39

3.32 ± 0.61

1.03 ± 0.15

χ11243

ΔrecA62

1.91 ± 0.69**

0.55 ± 0.20**

0.13 ± 0.03**

χ11244

ΔrecF126

5.00 ± 0.70

1.16 ± 0.21**

0.34 ± 0.04**

χ11245

ΔrecJ1315

2.56 ± 0.41

1.83 ± 0.99**

0.64 ± 0.15**


aIntraplasmid recombination without intervening sequence (5'tet-3'tet).

b Intraplasmid recombination with a 1041-bp intervening sequence (5'tet-kan-3'tet).

c Interplasmid recombination.

d Not assayed.

** P < 0.01, relative to the parental rec+ strain.

Zhang et al. BMC Microbiology 2011 11:31   doi:10.1186/1471-2180-11-31

Open Data