Table 4

Transformation with the HP1 knockout construct

Blast

Sclerotia


Experimental material

Mycelium1

Sclerotia

Quantity per experiment2

4

20

Transformants3

30%

15%


1On PDA plates.

2Number of plates used for blasting. Ten plugs were excised from each plate resulting in 40 isolates subjected to Hyg selection.

3Verified by Hyg selection and PCR.

Ish - Shalom et al. BMC Microbiology 2011 11:266   doi:10.1186/1471-2180-11-266

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