Table 3

Transformation with the pBC-bRPhleo construct

Blast

Sclerotia


Experimental material

Mycelium1

Sclerotia

Quantity per experiment2

10

120

Transformants3 (%)

34%

54%


1On PDA plates.

2Number of plates used for blasting. Ten plugs were excised from each plate resulting in 100 isolates subjected to Phleo selection.

3Verified by Phleo selection and PCR.

Ish - Shalom et al. BMC Microbiology 2011 11:266   doi:10.1186/1471-2180-11-266

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