Table 2

Transformation with the bR knockout construct

Blast

Sclerotia

Electroporation


Experimental material

Mycelium1

Sclerotia

Cells2

Quantity per experiment3

10

45

3 x106

Transformants4

39%

46%

0

Putative knockouts5

54%

62%

0


1On PDA plates.

2Protoplasts generated from broken hyphae, germinating conidia or both.

3Number of plates used for blasting. Ten plugs were excised from each plate resulting in 100 isolates subjected to Hyg selection.

4Verified by Hyg selection and PCR.

5Homologous recombination verified by PCR and sequencing.

Ish - Shalom et al. BMC Microbiology 2011 11:266   doi:10.1186/1471-2180-11-266

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