Effect of ΔpurR and Δfnr mutations on sensitivity to topoisomerase I cleavage complex Strains BW27784 and its isogenic derivatives IFL6 (Δfnr), IFL7 (ΔpurR) were transformed with pAYTOP128 and grown in LB with shaking to exponential phase before the addition of 0.002% arabinose. (a) Viable colony counts of arabinose treated cultures were divided by the colony counts from the untreated culture to obtain the survival ratio. (b) Western blot analysis showed that the ΔpurR and Δfnr mutations did not affect expression levels of mutant YpTOP after induction with 0.002% arabinose for 2.5 h.
Liu et al. BMC Microbiology 2011 11:261 doi:10.1186/1471-2180-11-261