Open Access Research article

Life without a cell membrane: Challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta)

Joke Hollants12*, Helen Decleyre1, Frederik Leliaert2, Olivier De Clerck2 and Anne Willems1

Author Affiliations

1 Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium

2 Phycology Research Group, Department of Biology, Ghent University, Krijgslaan 281 (S8), 9000 Ghent, Belgium

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BMC Microbiology 2011, 11:255  doi:10.1186/1471-2180-11-255

Published: 21 November 2011

Additional files

Additional file 1:

Transmission electron micrograph of vegetative Bryopsis thallus in longisection. Figure A: the outer cytoplasmic layer (ol) adjacent to the Bryopsis cell wall (cw) contains most of the organelles excluding only the chloroplasts (chl), which are present in the inner layer next to the central vacuole (cv). Magnification: × 8000, Scale bar: 3 μm. Figure B (detail of Figure A): besides mitochondria (m), endoplasmic reticulum and vacuolar evaginations (v), endogenous bacteria (ba) are present in the outer cytoplasmic layer. Magnification: × 25000, Scale bar: 1 μm.

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Additional file 2:

The marker used as a normalization and identification tool in all DGGE analyses. This marker covers the full range of endophytic (including chloroplast) sequences previously obtained from Bryopsis samples MX19, MX90, MX164, MX263 and MX344 [3]. For each marker band, the band name (M1m, M1b, M2-M10), taxonomic identification, clone reference and accession number are represented.

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