Table 1

Comparison of culture and qPCR for quantification of Legionella.

Number of positive samples

Concentrations


Culture

qPCR

Culture

qPCR


Sampling

Sampling site

Type of sample

No of samples

Legionella spp

Legionella spp

Legionella pneumophila

Legionella spp 104 CFU/L

Legionella spp104 GU/L

Legionella pneumophila 104 GU/L


Circulation water

B

1

1

1

1

5.5

3.4

3.6


Before the first intervention

Empty apartment

A

0


Shower hose

A

1

1

1

1

60

26

14


Circulation water

B

10

10

10

10

0.005 - 1.2

[0.08]

0.77 - 2.9

[1.5]

0.6 - 2.6

[1.1]


After the first intervention

Empty apartment

A

4

4

4

4

1.9 - 33

[19]

2.9 - 24

[8.9]

4.9 - 19

[11]


Shower hose

A

5

5

5

5

0.8 - 160

[27]

3.5 - 96

[28]

1.1 - 43

[17]


Circulation water

B

16

0

16

13

BD

0.4-1.9

[0.62]

BD - 2.0

[0.27]


After the second intervention

Empty apartment

A

2

1

2

2

BD - 0.001

3.2 - 55

[29]

3.7 - 68

[36]


Shower hose

A

8

0

8

8

BD

0.17 - 2.3

[0.95]

0.033 - 3.2

[1.3]


Number of samples and amount of Legionella detected in samples from circulation water, from first flush of taps in empty apartments and from first flush of shower hoses by culture and by Legionella pneumophila and Legionella species qPCR assays before and after interventions. Samples were collected as first flush (A) or after reaching constant temperature (B). BD: Below detection. Median value is given in [..]

Krøjgaard et al. BMC Microbiology 2011 11:254   doi:10.1186/1471-2180-11-254

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