Expression of emhABC efflux genes. Expression of emhABC in P. fluorescens strain cLP6a grown to stationary (Stat), logarithmic (Log) or Death phase at 28°C; grown to stationary phase at 10°C or 35°C; grown to stationary phase at 28°C in the presence of chloramphenicol (Chl) or tetracycline (Tet) at 1/4 MIC; or grown to stationary phase at 28°C in the presence of naphthalene (Nap) or phenanthrene (Phen) at 5 mmol l-1, determined using RT-qPCR. The values shown are the fold-difference in expression of emhABC compared to expression levels in cells grown to stationary phase at 28°C (calibrator = 1). Each bar represents the mean of two independent experiments performed in duplicate. Error bars, where visible, indicate the average deviation.
Adebusuyi and Foght BMC Microbiology 2011 11:252 doi:10.1186/1471-2180-11-252