Accumulation of low-RpoS mutants in LB-stabs. Ten LB-stabs were inoculated with a single colony of MC4100TF and incubated at room temperature. Every week two stabs were opened, the bacteria on the top of the medium was removed, diluted and plated in duplicates. Colonies were stained with iodine and counted.
Spira et al. BMC Microbiology 2011 11:248 doi:10.1186/1471-2180-11-248