Open Access Research article

Niche differentiation of two sympatric species of Microdochium colonizing the roots of common reed

Michael Ernst1, Karin Neubert1, Kurt W Mendgen1 and Stefan GR Wirsel23*

Author Affiliations

1 Lehrstuhl Phytopathologie, Fachbereich Biologie, Universität Konstanz, Universitätsstr. 10, D-78457 Konstanz, Germany

2 Institut für Agrar- und Ernährungswissenschaften, Naturwissenschaftliche Fakultät III, Martin-Luther-Universität Halle-Wittenberg, Betty-Heimann-Str. 3, D-06120 Halle (Saale), Germany

3 Interdisziplinäres Zentrum für Nutzpflanzenforschung, Martin-Luther-Universität Halle-Wittenberg, Betty-Heimann-Str. 3, D-06120 Halle (Saale), Germany

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BMC Microbiology 2011, 11:242  doi:10.1186/1471-2180-11-242

Published: 27 October 2011

Additional files

Additional file 1:

Details of isolates studied. This file provides a list of 21 Microdochium isolates used in this study, including accession numbers of ITS sequences and information about their origins.

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Additional file 2:

Specificity of nested-PCR assays targeting Microdochium spp. This file documents the specificity of the assays employed. A) First PCR step using primers ITS1F and ITS4. M = 100 bp size standard, water: no template DNA included, P. australis: genomic DNA of axenically grown reed plants, genomic DNAs from fungal isolates 4/97-9 (Humicola sp.), 6/97-38 (Chaetomium sp.), 6/97-54 (Fusarium sp.), A4 (Fusarium sp.), 5/97-16 (Microdochium phragmitis), 5/97-54 (M. bolleyi), 5/97-49 (M. bolleyi), 5/97-66 (M. phragmitis), respectively. B) and C) Second PCR steps using primers 5/97-16/ITS.F2 and 5/97-16/ITS.R2, and 5/97-54/ITS.F2 and 5/97-54/ITS.R2, respectively, and the products of the first PCR step as templates.

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Additional file 3:

Utilization of carbon sources. This file documents relative growth of Microdochium isolates on 95 different carbon sources on BIOLOG SF-N2 microtiter plates.

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Additional file 4:

Pair-wise analysis of spatial niche differentiation. This file includes P-values from binomial distribution tests for pair-wise analysis of occurrence between five fungal species from reed with respect to space and time. This data set was used to create Figure 5A and 5B.

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Additional file 5:

Pair-wise analysis of co-occurrence. This file includes P-values from Fisher's Exact tests for pair-wise analysis of co-occurrence between five fungal species from reed with respect to space and time.

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