Table 3 |
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|
Primers used in this study. |
|||
|
Primera |
Sequence (5'-3') |
Amplicon Size (bp) |
Reference |
|
|
|||
|
QacA/B_Fw |
GCTGCATTTATGACAATGTTTG |
628 |
[30] |
|
QacA/B_Rv |
AATCCCACCTACTAAAGCAG |
||
|
|
|||
|
Smr_Fw |
ATAAGTACTGAAGTTATTGGAAGT |
285 |
[18] |
|
Smr_Rv |
TTCCGAAAATGTTTAACGAAACTA |
||
|
|
|||
|
NorA_Fw |
TTCACCAAGCCATCAAAAAG |
620 |
[32] |
|
NorA_Rv |
CTTGCCTTTCTCCAGCAATA |
[13] |
|
|
|
|||
|
NorA_Fw |
TTCACCAAGCCATCAAAAAG |
95 |
[32] |
|
NorA_RT(Rv) |
CCATAAATCCACCAATCCC |
This study |
|
|
|
|||
|
NorB_Fw |
AGCGCGTTGTCTATCTTTCC |
213 |
[13] |
|
NorB_Rv |
GCAGGTGGTCTTGCTGATAA |
||
|
|
|||
|
NorC_Fw |
AATGGGTTCTAAGCGACCAA |
216 |
[13] |
|
NorC_Rv |
ATACCTGAAGCAACGCCAAC |
||
|
|
|||
|
MepA_Fw |
ATGTTGCTGCTGCTCTGTTC |
718 |
[13] |
|
MepA_Rv |
TCAACTGTCAAACGATCACG |
||
|
|
|||
|
MepA_RT(Fw) |
TGCTGCTGCTCTGTTCTTTA |
198 |
[13] |
|
MepA_RT(Rv) |
GCGAAGTTTCCATAATGTGC |
||
|
|
|||
|
MdeA_Fw |
AACGCGATACCAACCATTC |
677 |
[13] |
|
MdeA_Rv |
TTAGCACCAGCTATTGGACCT |
||
|
|
|||
|
MdeA_RT(Fw) |
GTTTATGCGATTCGAATGGTTGGT |
155 |
[33] |
|
MdeA_RT(Rv) |
AATTAATGCAGCTGTTCCGATAGA |
||
|
|
|||
|
16S_27f |
AGAGTTTGATCMTGGCTCAG |
492 |
[34] |
|
16S_519r |
GWATTACCGCGGCKGCTG |
||
|
|
|||
|
GrlA_Fw |
TGCCAGATGTTCGTGATGGT |
339 |
[35] |
|
GrlA_Rv |
TGGAATGAAAGAAACTGTCTC |
||
|
|
|||
|
GyrA_Fw |
TCGTGCATTGCCAGATGTTCG |
394 |
[35] |
|
GyrA_Rv |
TCGAGCAGGTAAGACTGACGG |
||
|
|
|||
|
aThe primers used in the RT-qPCR experiments are indicated by the RT label. Fw: forward; Rv: reverse. For norB, norC and smr, the same set of primers was used for both PCR and RT-qPCR, as well as the primer NorA_Fw. |
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|
Costa et al. BMC Microbiology 2011 11:241 doi:10.1186/1471-2180-11-241 |
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