Figure 3.

Inhibition of PI3K suppresses HSV-1-induced reactivation of KSHV. (A) RT-qPCR was used to detect relative quantities of ORF26 mRNA in LY294002 or DMSO control pretreated and HSV-1 infected BCBL-1 cells as indicated. ** p < 0.01 and *** p < 0.001 for Student's t-test versus Mock + DMSO group; ## p < 0.01 for Student's t-test versus HSV-1 + DMSO group. (B) Western blot analysis was used to detect the expression of KSHV vIL-6 and phosphorylated AKT in LY294002 or DMSO pretreated and HSV-1 infected BCBL-1 cells as indicated. (C) Western blot analysis was used to detect the expression of KSHV Rta, vIL-6 and phosphorylated GSK-3β in PI3K-DN or control vector transfected and HSV-1 infected BCBL-1 cells as indicated.

Qin et al. BMC Microbiology 2011 11:240   doi:10.1186/1471-2180-11-240
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