ALN, a cholesterol-dependent cytolysin, has hemolytic activity that is less sensitive to cholesterol inhibition than PFO. His-tagged CDCs were preincubated with dilutions of cholesterol for 30 min at room temperature prior to hemolytic assay. Abbreviations as in Figure 2. Error bars indicate one standard deviation from the mean calculated from the averages of three independent experiments conducted in triplicate.
Jost et al. BMC Microbiology 2011 11:239 doi:10.1186/1471-2180-11-239