Table 1

Strains and plasmid used in this study.

Strains or plasmids

Relevant phenotype or genotype

Reference


Strains

S. thermophilus

CNRZ368

Wild-type strain carrying ICESt1

INRA-CNRZ

CNRZ385

Wild-type strain carrying ICESt3

INRA-CNRZ

CNRZ368ΔICESt1

Wild-type strain cured from its ICESt1 resident element

X. Bellanger pers. com.

LMG18311 ICESt3cat

Wild-type strain carrying ICESt3 tagged with the cat gene inserted in the pseudogene Ψorf385J, Cmr

[10]

CNRZ368 ICESt3cat

CNRZ368ΔICESt1 strain carrying ICESt3cat, Cmr

This work

E. coli

DH5α

supE44 lacU169 (φ80 lacZ M15) hsdR17 endA1 gyrA96 thi-1 relA1

[33]


Plasmid

pSL1180

3, 4 kb, replication origin from pBR322, Ampr

Amersham


Abbreviations: Cmr, chloramphenicol resistance, Ampr: ampicillin resistance.

Carraro et al. BMC Microbiology 2011 11:238   doi:10.1186/1471-2180-11-238

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