Table 3

Newly Identified Genes Regulated by Fur That Contain a Predicted Fur Binding Site

Gene

Function

Fold Changea

Predicted Fur Binding Sequenceb


rlgA

Putative resolvase

2.8

AAAATTAAAATCGTTGGC

mapc

Methionine aminopeptidase

2.6

AAATTGAGAATCATTCTG

rpsB

30S ribosomal subunit protein S2

4.0

AAATTGAGAATCATTCTG

yajC

Tranlocase protein, IISP family

3.2

GTAATGCAAAGCATAAAA

nrdRc

Putative transcriptional regulator

2.5

GAAACGGTAAAAATTACC

sucC

Succinyl-CoA synthetase, beta subunit

4.1

CTAAAGATAACGATTACC

cmk

Cytidine monophosphate kinase

2.7

AAAAAGTAAATCATTGTC

STM1013

Gifsy-2 prophage, regulatory protein

2.8

AAAATCAAAATCAGTAAC

STM1133c

Putative dehydrogenase

-4.2

ATAATGAGTAGAATTGTT

nthc

Endonuclease III

2.9

GAAAAGCGTACCATTCCC

ldhAc

Fermentative D-lactate dehydrogenase

-4.0

AATATGCTTAAAATTATC

ynaFc

Putative universal stress protein

-37.3

GAAATAGATATAATTTAT

hns

Histone like protein

3.1

ACAATGCTTATCATCACC

STM1795c

Homolog of glutamic dehydrogenase

5.8

AAAAAGATAAAAATAACC

STM2186

Putative glutamate synthase

-8.8

AAATTGAGAATAGTTATT

eutCc

Ethanolamine ammonia lyase

-4.1

ATAATGCCCATCGTTTCC

eutBc

Ethanolamine ammonia lyase

-3.2

AAACTGATAAACATTGCC

yffBc

Putative glutaredoxin

2.6

GAAATTCGAATAAATAAT

iroNc

TonB-dependent siderophore receptor

9.1

CTAATGATAATAATTATC

yggUc

Cytoplasmic protein

3.5

ATAACGCTAAGAATAAAC

STM3600c

Putative sugar kinase

-6.8

CTGATGCTCATCATTATT

STM3690

Putative lipoprotein

-4.2

ATAAACATTATAATTATA

rpoZc

RNA polymerase, omega subunit

3.9

AATAAGATAATCATATTC

udpc

Uridine phosphorylase

-5.4

CAATAAATAATCAATATC

yjcDc

Putative xanthine/uracil permease

2.8

AAAAAGCAAACGATTATC

dcuA

Anaerobic dicarboxylate transport protein

-5.8

CAAATAACAACAATTTAA


a Ratio of mRNA, Δfur/14028s

b Predicted Fur binding site located within -400 to +50 bp relative to ATG

c Indicates the predicted Fur binding site is located on the reverse strand

Troxell et al. BMC Microbiology 2011 11:236   doi:10.1186/1471-2180-11-236

Open Data