Open Access Highly Accessed Research article

Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - a comparison of culture-dependent and culture-independent methods

Miia Pitkäranta1*, Teija Meklin23, Anne Hyvärinen2, Aino Nevalainen2, Lars Paulin1, Petri Auvinen1, Ulla Lignell2 and Helena Rintala23

Author Affiliations

1 Institute of Biotechnology, University of Helsinki, Viikinkaari 4, 00790 Helsinki, Finland

2 Department of Environmental Health, National Institute for Health and Welfare, Neulaniementie 4, 70701 Kuopio, Finland

3 Mikrobioni Inc., P.O. Box 1188, 70211 Kuopio, Finland

For all author emails, please log on.

BMC Microbiology 2011, 11:235  doi:10.1186/1471-2180-11-235

Published: 21 October 2011

Additional files

Additional file 1:

Fig. S1: Rarefaction curves for the analysed nucITS clone libraries.

Format: PDF Size: 216KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Table S1: Phylogenetic description, nearest database relative and frequency of detection of fungal molecular OTUs and isolated strains recovered from dust and water damaged building material.

Format: PDF Size: 177KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Table S2: List of fungal phylotypes obtained from building materials by cultivation and clone library analysis.

Format: PDF Size: 121KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Tables S3 and S4: Concentrations and diversity of fungi determined by culture (S3) and quantitative PCR (S4) in dust.

Format: PDF Size: 98KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Fig. S2: Comparison of clone library frequencies and qPCR cell counts for fungal phylotypes targeted by mold specific qPCR.

Format: PDF Size: 66KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 6:

Table S5: Statistical pair-wise comparison of nucITS clone libraries from settled dust samples.

Format: PDF Size: 54KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 7:

Table S6: List of performed qPCR assays and targeted species.

Format: PDF Size: 78KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 8:

Table S7: Summary of analysed samples and applied methods.

Format: PDF Size: 46KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data