Figure 2.

Screening of M. smegmatis mutant library. A) (Left panel) M. smegmatis wild type and ppk mutant were grown in M9 minimal medium supplemented with glucose 0.2% until OD600nm = 1.0, serially diluted up to 10-5 and transferred by using a metal replicator on agar plates. (Right panel) After incubation at 37°C for 4-5 days for aerobic cultures, or incubation for 2 weeks in an AnaeroGen gas pack system at 37°C followed by incubation under aerobic condition at 37°C for 4-5 days, plates were compared. B) Individual screening of 6 selected mutants. Each clone was grown in M9 minimal medium supplemented with glucose 0,2% until OD600nm = 1.0, serially diluted up to 10-5 and transferred by using a metal replicator on agar plates. Clones 1, 3 and 6 were considered as moderately affected clones. Clone 2 was considered as severely affected. ND = Non Diluted culture

Cordone et al. BMC Microbiology 2011 11:231   doi:10.1186/1471-2180-11-231
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