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Open Access Highly Accessed Research article

The rgg 0182 gene encodes a transcriptional regulator required for the full Streptococcus thermophilus LMG18311 thermal adaptation

Romain Henry12, Emmanuelle Bruneau12, Rozenn Gardan3, Stéphane Bertin12, Betty Fleuchot3, Bernard Decaris12 and Nathalie Leblond-Bourget12*

Author Affiliations

1 INRA, UMR1128 Génétique et Microbiologie, F-54506 Vandœuvre-lès-Nancy Cedex, France

2 Université de Lorraine, UMR1128 Génétique et Microbiologie, F-54506 Vandœuvre-lès-Nancy Cedex, France

3 INRA, UMR1319 MICALIS, Equipe Peptides et Communication Bactérienne, F-78352 Jouy en Josas, France

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BMC Microbiology 2011, 11:223  doi:10.1186/1471-2180-11-223

Published: 7 October 2011

Abstract

Background

Streptococcus thermophilus is an important starter strain for the production of yogurt and cheeses. The analysis of sequenced genomes of four strains of S. thermophilus indicates that they contain several genes of the rgg familly potentially encoding transcriptional regulators. Some of the Rgg proteins are known to be involved in bacterial stress adaptation.

Results

In this study, we demonstrated that Streptococcus thermophilus thermal stress adaptation required the rgg0182 gene which transcription depends on the culture medium and the growth temperature. This gene encoded a protein showing similarity with members of the Rgg family transcriptional regulator. Our data confirmed that Rgg0182 is a transcriptional regulator controlling the expression of its neighboring genes as well as chaperones and proteases encoding genes. Therefore, analysis of a Δrgg0182 mutant revealed that this protein played a role in the heat shock adaptation of Streptococcus thermophilus LMG18311.

Conclusions

These data showed the importance of the Rgg0182 transcriptional regulator on the survival of S. thermophilus during dairy processes and more specifically during changes in temperature.