Table 2

Correlation between the lesion diameters of the fruit samples, the amount of B. cinerea antigen determinated by the ELISA developed and the DNA of B. cinerea quantified from infected fruit extracts samples obtained at 4, 7, and 10 days of incubation (25°C), respectively.

Fruit samples

Days of incubation

bLesion diameters (mm/rot)

c B. cinerea antigen

(μg mL-1)

c DNA- B. cinerea

(μg mL-1)


Apples (Red-delicious)

a Control

uninfected

not detected

not detected

4

not visible

10.53 ± 0.48

10.22 ± 0.53

7

20.11 ± 0.54

40.67 ± 0.37

38.75 ± 0.41

10

50.09 ± 4.49

69.08 ± 0.43

71.19 ± 0.37

Table grapes (pink Moscatel)

a Control

uninfected

not detected

not detected

4

not visible

14.26 ± 0.51

13.86 ± 0.54

7

3.69 ± 0.52

49.03 ± 0.46

51.99 ± 0.42

10

5.35 ± 0.14

77.18 ± 0.36

75.84 ± 0.41

Pears (William's)

a Control

uninfected

not detected

not detected

4

not visible

11.29 ± 0.47

12.76 ± 0.51

7

15.13 ± 1.23

41.78 ± 0.55

41.44 ± 0.48

10

38.98 ± 1.67

70.84 ± 0.49

72.39 ± 0.52


a Negative control (uninfected fruits).

b Diameters of the lesion measured in the fruit samples at 4, 7 and 10 days of incubation (25°C) respectively.

b, c X (μg mL-1), mean ± SD, standard deviation.

Fernández-Baldo et al. BMC Microbiology 2011 11:220   doi:10.1186/1471-2180-11-220

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