Table 2

Previously published interactions among lambda proteins

interacting λ proteins

notes

ref#


head


1

A

Nu1

A (N-term) - Nu1 (C-term)

[32-34]

2

A

B

A (C-term) - B (= portal)

[32,35]

3

A

FI

Genetic evidence

[21]

4

FI

E

Genetic evidence

[22]

5

Nu3

B

Nu3 required for B incorporation into procapsid

[36]

6

W

B

[37,38]

7

W

FII

W required for FII binding, FII connects head to tail

[37,39]

8

B

B

12-mer (22 aa removed from B N-term)

[40,41]

9

C

E

Covalent PPI (in virion?)

[42,43]

10

C

B

[44]

11

B

E

copurify in procapsid

[45]

12

C

Nu3

C may degrade Nu3 (before DNA packaging)

[45-47]

13

D

D

Capsid vertices, D forms trimers

[48-50]

14

E

E

Main capsid protein

[20,51,52]

15

D

E

[20,51,52]

Nu3

Nu3

Nu3 multimer

unpublished *


tail


16

U

U

"probably a hexamer", interact in crystal

[53]

17

V

V

[51,54-56]

18

V

GT

the T domain binds soluble V

[24]

19

H

G/GT

G/GT hold H in an extended fashion

[24]

20

H

V

V probably assembles around H, displacing G/GT

[57]


replication


21

O

O

O-O interactions when bound to ori DNA

[58]

22

O

P

[59-62]


transcription


23

CI

CI

Forms octamer that links OR to OL

[63,64]

24

CII

CII

homotetramers

[65]

25

CIII

CIII

dimer

[66]

26

Cro

Cro

dimer; x-ray structure

[67]


Recombination


27

Exo

Bet

[68]

28

Xis

Int

[69] #

29

Xis

Xis

Xis-Xis binding mediates cooperative DNA-binding

[69] #

30

Int

Int

Dimer

[70]


lysis


31

Rz

Rz1

heteromultimer that is supposed to span the periplasm

[71]

32

S

S

large ring in inner membrane

[72]

S

S'

S' inhibits S ring formation (S: 105 aa, S': 107 aa)

[73]


lysogenic conversion


33

SieB

Esc

Esc is encoded in frame in sieB + inhbits sieB

[74,75] #


bold: found in this study. * unpublished (C. Catalano, pers. comm., by permission), # interactions not tested in Y2H assays (one or both clones not available).

Rajagopala et al. BMC Microbiology 2011 11:213   doi:10.1186/1471-2180-11-213

Open Data