Table 2

Bacterial strains and plasmids

Strain or plasmid

Relevant characteristic(s)

Source or reference


E. coli strains

536 wt

UPEC wild type strain, SmR

[69]

536-21

536, ΔPAI I536 ΔPAI II536, SmR

[2]

536-19/1mob

Donor strain in the mobilisation experiments, pirλatt, mobGP704 inserted in PAI II536, pRP4, SmR, ApR, CmR, TcR, KmR

This study

SY327λpir

F-, araD, Δ(lac pro), argE(Am), recA56, RifR, gyrA λpir

[60]

SM10λpir

thi1, thr1, leuB6, supE44, tonA21, lacY1, recA::RP4-2-Tc::Mu λpir KmR

[60]

SY327-23

Mobilised PAI II536 is integrated into leuX

This study

SY327-77

Mobilised PAI II536 is present as a CI

This study

Plasmids

pGEM®T-Easy

bla, T/A cloning vector

Promega

pGP704

bla, oriR6K, mobRP4

[60]

pSG704

cat, oriR6K, mobRP4

This study

pCVD442Tc

bla, tet, oriR6K, mobRP4, sacB

This study

pLDR8 neo, int expression vector, Ts

neo, int expression vector, Ts

[62]

pLDR9

bla neo, cloning vector to integrate DNA into attB

[62]

pPAI II-CI

bla, positive control for detection of PAI II536-specific CIs

[17]


Schneider et al. BMC Microbiology 2011 11:210   doi:10.1186/1471-2180-11-210

Open Data