Table 1

Mobilisation and remobilisation of PAI II536

Transfer rate of PAI II536

20°C

37°C


    Mobilisation rate from
    E. coli
    536 to
    E. coli
    SY327

Donor-recipient ratio 3:1

3.47 × 10-08 ± 4.85 × 10-09

3.65 × 10-08 ± 5.46 × 10-09

Donor-recipient ratio 9:1

4.93 × 10-08 ± 1.14 × 10-08

4.31 × 10-08 ± 6.11 × 10-09

    Remobilisation rate from
    E. coli
    SY327 to
    E. coli
    536-21

Donor with integrated PAI II536

1.41 × 10-07 ± 1.25 × 10-07

8.00 × 10-08 ± 7.47 × 10-08

Donor with CI of PAI II536

4.32 × 10-05 ± 3.65 × 10-05

3.75 × 10-05 ± 3.18 × 10-05


31 and 10 independent conjugation experiments were performed for the mobilisation and remobilisation experiment, respectively. Plasmid RP4 was used as a helper plasmid for mobilisation of the excised PAI II536 construct from E. coli 536 into recipient E. coli SY327.

Schneider et al. BMC Microbiology 2011 11:210   doi:10.1186/1471-2180-11-210

Open Data