EscU or EscU variants from EPEC lysates do not co-purify with immunoprecipitated CesT. Cell lysates were generated from the indicated bacterial strains and exposed to anti-CesT antibodies in a co-immunoprecipitation experiment. The lysate inputs were probed with the indicated antibodies (top panel). Anti-RNA polymerase antibodies were used to detect RNA polymerase amounts within the lysates which are expected to be equivalent. The elution fractions were probed with the indicated antibodies. tir and cesT null mutants were included as control strains in the experiment. Note that Tir is unstable in the absence of CesT and therefore was not detected in the elution fraction. The lane designations apply to all the panels.
Thomassin et al. BMC Microbiology 2011 11:205 doi:10.1186/1471-2180-11-205